50X TAE Buffer - DNA Electrophoresis Applications

TAE provides lower buffering capacity and conductivity than TBE, yielding sharper bands for plasmid DNA and PCR product analysis.

Molecular biology labs dilute to 1X (40mM Tris, 1mM EDTA) for routine 0.8-1.5% agarose gels resolving 100bp-5kb fragments.

Acetate buffer migrates faster than borate reducing run times while maintaining resolution for routine cloning QC.

Suitable for DNA recovery from gels due to easy passive elution compared to TBE.