Direct Bilirubin - Obstructive Pattern Applications

Water-soluble bilirubin diazo at 570nm confirms intrahepatic cholestasis preventing unnecessary ERCP. T/D>0.5 ratio guides ursodiol therapy.

Clinical laboratories exclude Gilbert syndrome through normal direct fraction. Accelerator-free prevents false elevation artifacts.

Quality control confirms conjugation specificity, hemolysis resistance, and light-stable formulation.