Bradford Assay - Protein Quantitation Applications

595nm dye shift (cationic?anionic protein binding) provides rapid microplate protein determination across Western blot loading optimization.

Research laboratories standardize 10-100 ?g/lane preventing overloading artifacts. Detergent compatibility prevents lysis buffer interference.

Quality control confirms dye purity, acid stability, and linear calibration curve. Micro-assay prevents macro-volume waste.