Carbol Fuchsin - TB Diagnosis Applications
Ziehl-Neelsen protocol: 60°C 5min staining → acid-alcohol decolorization → methylene blue counterstain reveals red acid-fast bacilli.Microbiology labs confirm M. tuberculosis (1:60 Kinyoun modification) distinguishing from Nocardia (partially acid-fast).0.3% basic fuchsin penetrates mycolic acids binding irreversibly preventing decolorization by 3% HCl-ethanol.Histology stains Helicobacter pylori (toluidine blue carbol fuchsin) in gastric biopsies.
