Catalase Test - Bacterial Differentiation Applications

Catalase-positive: immediate bubbling (Staphylococcus vs Streptococcus, Enterobacteriaceae vs anaerobes).

Microbiology laboratories confirm coagulase-negative Staphylococci vs Streptococci preventing methicillin misclassification.

Slide test: loopful organism + H?O? drop ? vigorous bubbling = catalase+ve; no reaction = catalase-ve (Streptococcus, Enterococcus).

Tube test differentiates Bacteroides (negative) from Prevotella (variable) in anaerobic identification.