Crystal Violet - Gram & Capsule Applications

Gram stain: 60s primary stain ? iodine mordant ? acetone decolorizer ? safranin counterstain differentiates Gram+ (purple) vs Gram- (pink).

Microbiology labs identify Gram+ cocci (Staphylococcus/Streptococcus) vs Gram- rods (Enterobacteriaceae/Pseudomonas).

Capsule stain (Hiss method): negative staining reveals acapsular halo around Cryptococcus neoformans, Klebsiella pneumoniae.

Biofilm crystal violet assay quantifies bacterial adhesion in 96-well plate antimicrobial screening.