Crystal Violet - Gram & Capsule Applications
Gram stain: 60s primary stain → iodine mordant → acetone decolorizer → safranin counterstain differentiates Gram+ (purple) vs Gram- (pink).Microbiology labs identify Gram+ cocci (Staphylococcus/Streptococcus) vs Gram- rods (Enterobacteriaceae/Pseudomonas).Capsule stain (Hiss method): negative staining reveals acapsular halo around Cryptococcus neoformans, Klebsiella pneumoniae.Biofilm crystal violet assay quantifies bacterial adhesion in 96-well plate antimicrobial screening.
