Methylene Blue - Microbiology Applications

Methylene blue stains bacterial cytoplasm blue in simple stains and Loeffler's methylene blue for Corynebacterium diphtheriae screening.

Laboratories use simple methylene blue wet mounts for motility (Treponema pallidum darkfield confirmation) and capsule visualization.

Field's Stain A component stains malaria chromatin red-purple differentiating ring forms from platelets.

Supravital stain reveals reticulofilaments in bone marrow confirming erythropoietic recovery post-chemotherapy.