Muller Hinton Blood Agar - Fastidious AST Specialist

Muller Hinton Agar with 5% defibrinated sheep blood combines standardized antimicrobial susceptibility testing performance with enriched nutrition for fastidious pathogens. The blood supplementation provides essential hemin (X-factor) and NAD (V-factor) while maintaining low sulfonamide/thymidine levels critical for predictable antibiotic diffusion and zone size interpretation.

Dual Clinical Applications

1. Fastidious Pathogen AST: Disk diffusion testing of beta-hemolytic streptococci, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis per CLSI M100 standards.

2. Primary Isolation: Enhanced recovery from respiratory specimens with hemolytic reaction visualization.

3. Quality Control: QC medium for antibiotic disks targeting fastidious organisms.

Technical Superiority

19mm x 15mm x 20ml plates (Cat. 0801100E), 10 pcs/stack. SFDA compliant, ISO 13485 manufactured. Uniform blood distribution, controlled thymidine levels, optimal pH stability (7.2-7.4).

QC strains: S. pneumoniae ATCC 49619, H. influenzae ATCC 49247, E. coli ATCC 25922.

Laboratory Protocol

Prepare 0.5 McFarland suspension. Lawn inoculation, disk placement within 15 minutes. Incubate 35°C, 5% CO2, 20-24h for streptococci. Measure zones per CLSI tables with hemolytic pattern documentation.