Phosphate Buffered Saline pH 7.2 - Cell Biology Applications

PBS (137mM NaCl, 2.7mM KCl, 10mM phosphate, pH 7.2) maintains physiological conditions supporting mammalian cell manipulation across research laboratories.

Cell culture laboratories wash adherent cells using ice-cold PBS preventing receptor internalization. Physiological osmolarity maintains membrane integrity while calcium/magnesium-free formulation facilitates trypsin dissociation.

Flow cytometry laboratories employ PBS-FCS suspension media maintaining cell viability during antibody staining. Low autofluorescence prevents spectral spillover compromising multi-color panel compensation accuracy.

Immunohistochemistry laboratories dilute antibodies in PBS-Tween preventing non-specific binding. Physiological pH maintains epitope integrity while detergent reduces hydrophobic interactions compromising signal specificity.

Quality control verifies osmolarity (280-320 mOsm/kg), endotoxin (<0.1 EU/mL), and sterility assurance. 10X concentrate enables one-step dilution eliminating weighing inconsistencies across users.

0.2?m sterile filtration prevents microbial contamination during extended protocols. Protease-free formulation maintains cytokine stability during supernatant collection and storage.

Storage prevents phosphate precipitation through 15-30°C conditions. Calcium-compatible formulation supports live/dead discrimination assays requiring divalent cation signaling.

Safety classification as non-hazardous material simplifies shipping and disposal. Secondary containers prevent cross-contamination between cell culture workstations and analytical instruments.

ISO 13485 manufacturing confirms phosphate molarity, chloride content, and divalent cation absence. Validation studies verify performance equivalence supporting cell biology laboratory accreditation.