Safranin - Gram & Spore Applications

Gram stain final step stains decolorized Gram-negatives red contrasting purple Gram-positives.

Microbiology labs perform Wirtz-Conklin spore stain: malachite green ? safranin reveals green spores, red vegetative cells.

Acid mucin histochemistry differentiates sulfated (red) vs carboxylated (blue) glycosaminoglycans.

DNA binding reveals bacterial chromatin in simple stains preventing crystal violet overload.