NaHCO? - Cell Culture & pH Applications

Sodium bicarbonate maintains physiological pH (7.2-7.4) in 5-10% CO? incubators supporting mammalian cell growth.

Laboratories prepare 7.5% stock solutions yielding 2.0-2.2 g/L working concentrations in DMEM and RPMI media.

It neutralizes acidic solutions and precipitates proteins in extraction protocols while serving as leavening agent in microbial media prep.

Effervescent properties aid in cleaning glassware and removing proteinaceous deposits.